Molecular characterization and analysis of antimicrobial. Antimicrobial assay thirty nine isolates of endophytic fungi were randomly chosen to estimate their ability to produce antimicrobial compounds antibacterial or antifungal activity. Two new fusicoccane diterpenes, named periconicins a 1 and b 2, with antibacterial activities have been isolated by bioassayguided fractionation from an endophytic fungus periconia sp. After which they were surface sterilized by submerging them in 75% ethanol for 2 min. Antibacterial activity of extract of endophytic fungi of. Screening of endophytic fungi for antimicrobial activity by agar well diffusion. The agnps produced by endophytic fungi displayed considerable antibacterial activity and hence this study would prove to. Endophytic fungithe treasure chest of antibacterial substances. The antibacterial activity of the etoac extracts of the selected endophytic fungi were also detected according to the previous report.
Gao 1school of plant protection, anhui agricultural university, hefei, china 2school of life science, anhui agricultural university, hefei, china. Endophytic fungi cultures were transferred to petri dish which consisted of nutrient agar medium and patogenic bacteria. Cinnamomum mercadoi is a medicinal tree endemic to the philippines. Antibacterial activity of endophytic fungi isolated from the bark of. The estimation was done on four bacteria species by disc diffusion method, which were incubated at 37 c for 24 hours and inhibition zones were measured. Antibacterial activities of fungal endophytes associated with. It is noteworthy that apart from being rich sources of secondary metabolites, these endophytic fungi also have the ability to reduce metals. Antibacterial activity of endophytic fungus, griseofulvum. Biosynthesis of silver nanoparticle by endophytic fungi. Endophytic fungi have the ability to coexist with their host plants without causing any harm and are beneficial to both the plant and the fungi. Biosynthesis, characterization and antibacterial activity. The current study determined the antimicrobial properties and identify the chemical compounds of secondary metabolites produced by endophytic fungi isolated from sceletium tortuosum l.
Antibacterial activity in higher fungi mushrooms and endophytic fungi from slovenia. Antibacterial and antifungal potential of endophytic fungi. The use of endophytic fungi for nanoparticles production remains untouched. Antimicrobial activity of endophytic fungi isolated and. Method endophytic fungi were isolated from the stem, leaves and root of angelica sinensis by the surface sterilization method and scoring method. Two new anisic acid derivatives from endophytic fungus. Introduction mangrove is a plant found in tidal areas such as coast of. Ophiopogon japonicus, containing compounds with antibacterial activity, is a traditional. It is a well known plant which possesses diverse health and industrial benefits. Endophytic fungi from the chinese medicinal plant actinidia macrosperma were isolated and identified for the first time. Antimicrobial activity in cultures of endophytic fungi isolated from. Five different taxa were represented by 11 isolates, and six isolates were.
Antibacterial activity of endophytic bacillus safensis isolated from ophioglossum reticulatum l. Thus, a varied range of natural products of plant, fungi and lichen origin were tested against two bacterial species, staphylococcus aureus atcc 25923 and escherichia coli atcc 25922, by. Endophytic fungi, mangrove, kurz, antibacterial activity. Among all endophytic fungi, exhibited prominent antibacterial activity whereas 4 did not exhibit any antibacterial activity. Antibacterial and antioxidant potential of endophytic fungi. After incubation, fermentation broth and biomass were extracted by ethyl acetate, nbothanol and ethanol. Paul1 1microbiology laboratory, department of botany, university of calcutta, kolkata, india. Antibacterial activities of fungal endophytes associated. Antibacterial and antioxidant potential of endophytic.
The fermentation broth was then removed, and the filtrate was extracted with 25. The fungal culture was extracted with ethyl acetate and used as a crude extract for checking antimicrobial activities by well diffusion method. Antimicrobial activity of endophytic fungi isolated from. Ophiopogon japonicus, containing compounds with antibacterial activity, is a. Zone of inhibition diameter in mm 300gwell among the endophytic flora, colletotrichum was found to be the most prominent genus. Periconicins, two new fusicoccane diterpenes produced by an.
Among 65 endophytic fungi isolated, 18 fungi showed activity against at least one tested microorganism in preliminary screening, and the best results were obtained with. Endophytic fungi that showed antimicrobial activity were identified based on analysis of the dna sequences of the its15. The purpose of the present study was to extract, explore and characterize antimicrobial activity produced by the endophytic fungi isolated from salt marsh plant leaves and stems of suaeda maritima and suaeda monoica. Briefly, pure cultures of the endophytic fungi were cultivated on the surface of pda at 28c for 14 days. The screening results revealed antagonistic properties for.
In vitro antioxidant and antibacterial activity of endophytic. Antimicrobial activity of endophytic fungi isolated and identified from. Rhizopycnis acids a 1 and b 2, two new anisic acid derivatives, were obtained from the ethyl acetate extract of the fermentation cultures of rhizopycnis vagum, an endophytic fungus isolated from the healthy tissues of nicotiana tabacum. Twenty fungal isolates were recovered from different parts of the host plant and they were characterized for their morphological features through. Antimicrobial activity of endophytic fungi from leaves and barks of. Endophytic fungi were isolated from surface sterilized bark samples aseptically. Frontiers antibacterial activity of endophytic fungi from.
Antibacterial activity of endophytic safensis isolated from. The present study is aimed at the isolation and identification of endophytic fungi from p. Antibacterial activity of endophytic fungi isolated from the. This claim was supported by the findings of a group of chinese researchers who isolated endophytic fungi from several chinese medicinal plants, and further tested the. The aim of this study was to identify the endophytic fungi from medicinal plant ricinus communis and to investigate their potential antibacterial activity. Antibacterial activity and preliminary phytochemical. Periconicins, two new fusicoccane diterpenes produced by.
Antibacterial activity antibacterial activities of the isolated endophytic fungi were checked against selected human pathogens escherichia coli, klebsiella pneumoniae, proteus vulgaris, pseudomonas aeruginosa, bacillus subtilis, staphylococcus aureus. Antimicrobial activity of crude extracts of endophytic. Pdf antibacterial activity of endophytic fungi isolated from conifer. Antibacterial activity of total flavonoids from endophytic fungal crude extraction.
Antibacterial activity of endophytic fungi from leaves of indigofera. The effect of the 26 endophytic fungi on the growth of p. In total, 377 out of 1979 endophytic fungi were included. Pdf endophytic fungi were isolated from healthy leaves of indigofera suffruticosa miller, a medicinal plant found in brazil which is used in folk. Evaluation of antimicrobial activity of endophytic fungi from. Endophytic fungi were isolated from healthy leaves of indigofera suffruticosa miller, a medicinal plant found in brazil which is used in folk medicine to treat various diseases.
Biosynthesis, characterization and antibacterial activity of. The purpose of the present study was to extract, explore and characterize antimicrobial activity produced by the endophytic fungi isolated from salt marsh plant leaves. Diversity estimation and antimicrobial activity of culturable. Five different taxa were represented by 11 isolates, and six isolates were grouped. Jan 02, 2016 several groups 11, 12, 18 have previously reported the antifungal activities of plant endophytic fungi and actinomycetes. Isolated endophytes were screened for their antibacterial activity against pathogenic bacteria. Isolation and antibacterial activity of endophytic fungi. Pdf antibacterial activity of endophytic fungi from leaves of. Pdf fungi, in particular endophytes are a promising source of new antimicrobial compounds. The systemic study was made on endophytic fungi of medicinal plant, g. Cinnamomum species have been extensively studied for their endophytic fungi with pharmacological importance.
A total of 60 endophytic fungi produced secondary metabolites. Virginia medeiros siqueira, raphael conti, janete magali araujo, cristina maria souzamotta. Antimicrobial activity and phytochemical analysis of crude. Production of bioactive secondary metabolites from endophytic. Production of bioactive secondary metabolites from. It also confirmed that the antimicrobial activity is attributable to the presence of endophytic fungi in the plant. Research article open access antimicrobial activities of. The first research, which proved the antibiotic activity of endophytic fungi, was.
In vitro antioxidant and antibacterial activity of. Among the 26 endophytic fungi, the inhibition of most strains varied from 6. The antibacterial activity as shown in table 1 and fig. The structures of the two compounds were determined through a series of 1d and 2d nmr and hrms spectral analyses. This study shows that the endophytic fungi isolated. All endophytes exhibited antibacterial activity on at least one of the test patho.
Results of the antibacterial assay showed that 10 mgml of the colletotrichum sp. Endophytes provide an abundant reservoir of bioactive metabolites for medicinal exploitation, and an increasing number of novel compounds are being isolated from endophytic fungi. The symbiotic relationship among endophytic fungi as well as plants gives endophytes the powerful capability to produce new bioactive substances 4,28. Isolated endophytes were screened for their antibacterial activity against an array of pathogenic bacteria. The structures of the new compounds were determined by combined spectroscopic methods. A total of 60 endophytic fungi produced secondary metabolites that were detected after fermentation and extraction. Dec 27, 2014 endophytic fungi were isolated from gymnema sylvestre, a known medicinal plant for hypoglycemic activity. The screening of the endophytic fungi f or antibacterial activity of their metabolites was performed by an antagonism test 14. Pdf antibacterial activity of endophytic fungi isolated. This study is the first report about the antimicrobial activity of endophytic fungi residing in i. Isolation and identification of flavonoidproducing. An increasing number of compounds with antibacterial activity are being isolated from endo. Among them were 80 morphologically distinct fungi from g. Objective to research antibacterial activity of limonium aureum endophytic fungi.
Methods the endophytic fungi of limonium aureum root was isolated by general method. It also leads to the justification that the studies on isolation and identification of the bioactive compounds can be an important and crucial approach to search for novel natural products. Several groups 11, 12, 18 have previously reported the antifungal activities of plant endophytic fungi and actinomycetes. Antimicrobial activity of endophytic fungi isolated. Extracts of cultured endophytic strains were tested for antimicrobial properties using a microdilution assay. Antimicrobial activity of endophytic fungi associated with orchidaceae in brazil. No reports are available on antimicrobial activities of the endophytic fungi isolated from e. The aim of this study was focused on the antibacterial activity of endophytic fungi from leaf, bark and root of mangrove sonneratia grifithii kurz, collected from bungus, west sumatra. Briefly, pure cultures of the endophytic fungi were cultivated on. Jy2 displayed antagonism activity against some pathogenic bacteria owing to the existence of antimicrobial compounds. Ananya mukherjee1, rituparna das1, arundhati pal2 and a. Antimicrobial activities of endophytic fungi isolated from. Pfe31 crude extract exhibited broadspectrum antibacterial activity against representative grampositive, gramnegative, and sporeforming bacilli.
Therefore, it could be suggested that the antibacterial activities exhibited in mangrove leaf extracts were probably contributed by the endophytic fungi living in them. Antibacterial activity of endophytic safensis isolated. Diversity and characterization of endophytic fungi isolated. Evaluation of antimicrobial activity of endophytic fungi. This work ins of sonneratia griffithii kurz can be developed as a new source of antibiotic compounds. Antibacterial activity in higher fungi mushrooms and. The results show that none of the treatments equaled 100 mgl carbendazim, which was used as the positive control. Frontiers antibacterial activity of endophytic fungi. Antibacterial activity of endophytic fungi isolated from sceletium. Diversity and characterization of endophytic fungi. Preliminary antibacterial assay antibacterial activity of the endophytic fungi was performed using the method of agar plug diffusion explained by powthong et al. Antibacterial activity of endophytic fungi isolated from. Isolation of endophytic fungi was based on direct planting method. Fungi are the most commonly isolated endophytic microbes.
Drug resistance in bacteria has become a global concern and the search for new antibacterial agents is urgent and ongoing. Dis paper diffusion method was adopted for the determination of the inhibition zone diameter against animal pathogenic bacteria, and the plateculture mediumcoating method was adopted for the determination of. This study aims to isolate and evaluate the antibacterial activity of endophytic fungi associated with the bark of c. Ophiopogon japonicus, endophytic fungi, peptide deformylase, antimicrobial activity background endophytes are microorganisms that live in the intercellular spaces of healthy host tissues without causing obvious symptoms 1. Identification of isolated endophytic fungi the endophytic fungal strains were identified based on morphology shape, colony, color, topography and microscopically following the protocol.
The bactefiostatic test was experimented with six different bacterium. The remaining fungal extracts exhibited significant level of total phenolic and flavonoid content, antioxidant and antibacterial activity. Antimicrobial activity in cultures of endophytic fungi. Endophytic fungi from medicinal plants of virudhunagar district for antimicrobial activity 148 isolation of endophytic fungi leaves were thoroughly washed with mild detergent and running tap water and then airdried. Endophytic fungi were isolated from healthy leaves of indigofera suffruticosa miller, a medicinal plant found in brazil which is used in folk. Isolation and antibacterial activity of endophytic fungi from. Antibacterial activity test the agar well diffusion assay method was used to examine the antibacterial activity of fungi. Pdf screening of endophytic fungi from chlorophyta and. The antibacterial extract was fractionated and purified using silica gel chromatography. Endophytic fungi from the medicinal plant lippia sidoides cham. Study on angelica sinensis endophytic fungi and its.
Thus, a varied range of natural products of plant, fungi and lichen origin were tested against two bacterial species, staphylococcus aureus atcc 25923 and escherichia coli atcc 25922, by two variants of the agar diffusion. Isolation and identification of endophytic fungi from. The filtrate revealed the strongest in vitro antimicrobial activity on pseudomonas aeruginosa by increasing the permeability of cell membranes. Endophytic fungi were isolated from gymnema sylvestre, a known medicinal plant for hypoglycemic activity. Endophytic fungi cut off a large number of metabolites live.
Endophytic fungi are an excellent source of secondary metabolites as a natural product 3. Antimicrobial activity of endophytic fungi associated with. Endophytic fungi strains that have sonneratia griffithii kurz. Endophytic fungi from the root tubers of medicinal plant.
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